| Gene name |
SPBC3E7.02c |
| Gene ID |
31/D08 |
| Gene synonyms/obsolete |
hsp16 |
| Gene product |
heat shock protein 16;
Hsp20 family; expression in response to nucleotide depletion
is regulated via the spc1/MAPK pathway; similar to Sp
SPCC338.06c |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
432 |
| ORF length (spliced) |
|
| Entry clone length |
432 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC3E7.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTTGCAACCTTTTTT |
| Rev primer name |
SPBC3E7.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTAATAGCAATTTGTTTC |
| Amino acid length |
143 |
| Molecular weight |
15.9 |
| Isoelectric point (calc.) |
5.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
strong signal of
aggregates by over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |