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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC14C8.12
Gene ID 31/E02
Gene synonyms/obsolete rpb8
Gene product DNA-directed RNA polymerase (I, II, and III subunit); involved in transcription from Pol I, II, and III promoter; DNA-directed RNA polymerase activity; interacts physically with Rpb1p, Rpb3p, Rpb11p, and Rpb6p
Entry clone Cloned#
ORF length (unspliced) 485
ORF length (spliced) 378
Entry clone length 485
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC14C8.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGGAATCCGTACTTTT
Rev primer name SPBC14C8.12.Rv
Rev primer SEQ AGAAAGCTGGGTACCTTCTAAGTAATAGATAA
Amino acid length 125
Molecular weight 14.3
Isoelectric point (calc.) 5.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol; nucleolus as dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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