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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3G9.03
Gene ID 31/E06
Gene synonyms/obsolete rpl2301; rpl23-1; rpl23
Gene product 60S ribosomal protein L23
Entry clone Cloned#
ORF length (unspliced) 531
ORF length (spliced) 420
Entry clone length 531
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC3G9.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTCGTGGACGCGGTGC
Rev primer name SPAC3G9.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAACTACAGTACCGGCATTA
Amino acid length 139
Molecular weight 14.8
Isoelectric point (calc.) 10.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion; nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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