Gene name |
SPBC32F12.09 |
Gene ID |
31/G12 |
Gene synonyms/obsolete |
rum1 |
Gene product |
CDK inhibitor;
regulator of G1 phase progression; involved in pheromone
induced G1 arrest; functional ortholog of Sc SIC1; 2
transcripts; expression cell cycle dependent; phosphorylated
by MAPK |
Entry clone |
Cloned |
ORF length (unspliced) |
693 |
ORF length (spliced) |
|
Entry clone length |
693 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC32F12.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACCTTCAACACCACC |
Rev primer name |
SPBC32F12.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCGTAATAAATTGTGCCTG |
Amino acid length |
230 |
Molecular weight |
25.2 |
Isoelectric point (calc.) |
10 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
nuclear dots by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |