Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22H12.01c
Gene ID 31/H01
Gene synonyms/obsolete SPAC23G3.13c
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 705
ORF length (spliced)
Entry clone length 705
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC22H12.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTAACGAAAGACATGA
Rev primer name SPAC22H12.01.Fd
Rev primer SEQ AGAAAGCTGGGTAAAAATGCGTTGGAATCACT
Amino acid length 234
Molecular weight 27.5
Isoelectric point (calc.) 5.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 4 images) See all images

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