Gene name |
SPBC36B7.07 |
Gene ID |
31/H10 |
Gene synonyms/obsolete |
tlg1 |
Gene product |
SNARE; involved in
secretory pathway; GPI anchored protein; 1 predicted
transmembrane helix; Belongs to the syntaxin/epimorphin
family; Contains 1 t-SNARE coiled-coil homology domain |
Entry clone |
Cloned |
ORF length (unspliced) |
769 |
ORF length (spliced) |
678 |
Entry clone length |
769 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC36B7.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGGACCCCTTTTATGA |
Rev primer name |
SPBC36B7.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAGCACAATAACCAATACC |
Amino acid length |
225 |
Molecular weight |
24.9 |
Isoelectric point (calc.) |
4.2 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIIILIALLVL |
Localization (YFP) |
ER; Golgi |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |