Gene name |
SPAC8C9.08 |
Gene ID |
31/H12 |
Gene synonyms/obsolete |
rps5 |
Gene product |
40S ribosomal protein
S5 |
Entry clone |
Cloned |
ORF length (unspliced) |
828 |
ORF length (spliced) |
612 |
Entry clone length |
828 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC8C9.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTACGTCGAGTCTCAC |
Rev primer name |
SPAC8C9.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACGGTTACTCTTGGCAACA |
Amino acid length |
203 |
Molecular weight |
22.2 |
Isoelectric point (calc.) |
10.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRRVNQALAL |
Localization (YFP) |
cytosol; occasionally
nucleolus |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>>cytosol |
Microscope used for
observation |
Leica |