Gene name |
SPBC725.02 |
Gene ID |
32/A07 |
Gene synonyms/obsolete |
mpr1; spy1 |
Gene product |
stress response
regulator phosphotransmitter; histidine-containing
phosphotransfer domain; respone regulator phosphotransferase;
interacts physically with Mcs4p; regulates G2/M progression
through the Mcs4 response regulator |
Entry clone |
Cloned |
ORF length (unspliced) |
888 |
ORF length (spliced) |
|
Entry clone length |
888 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC725.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTGTATATCGTGATAA |
Rev primer name |
SPBC725.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGTAGAAGAATTTTTTTCA |
Amino acid length |
295 |
Molecular weight |
32.5 |
Isoelectric point (calc.) |
4.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |