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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC530.10c
Gene ID 32/B08
Gene synonyms/obsolete anc1
Gene product adenine nucleotide carrier; mitochondrial carrier
Entry clone Cloned
ORF length (unspliced) 969
ORF length (spliced)
Entry clone length 969
No. of intron 0
Sequence status Finished
Sequence results 481G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC530.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTACTTCATCTGCTGC
Rev primer name SPBC530.10.Rv
Rev primer SEQ AGAAAGCTGGGTAGAACTTCTTGCCGAACATC
Amino acid length 322
Molecular weight 35
Isoelectric point (calc.) 10.4
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 3
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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