| Gene name |
SPBC17A3.10 |
| Gene ID |
32/B11 |
| Gene synonyms/obsolete |
pi036 |
| Gene product |
zinc finger protein;
zf-C3HC4 type (RING finger); ubiquitin ligase (E3); involved
in peroxisome assembly |
| Entry clone |
Cloned |
| ORF length (unspliced) |
979 |
| ORF length (spliced) |
921 |
| Entry clone length |
979 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
861C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC17A3.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCACTTGTCAGCACACAT |
| Rev primer name |
SPBC17A3.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCGGAGTAAAATGATTTTT |
| Amino acid length |
306 |
| Molecular weight |
34.3 |
| Isoelectric point (calc.) |
8.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSAHIDPLQI/LVSIIRFLCL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |