| Gene name |
SPBC19F8.07 |
| Gene ID |
32/C10 |
| Gene synonyms/obsolete |
mop1; mcs2; crk1 |
| Gene product |
serine/threonine
protein kinase; cyclin-dependent kinase activating kinase
(CAK); mitotic catastrophe suppressor; activator of Cdc2p;
transcription initiation factor TFIIH subunit (RNA polymerae
II CTD kinase) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1008 |
| ORF length (spliced) |
|
| Entry clone length |
1008 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
346A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC19F8.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATATCGAAAAGTCTGA |
| Rev primer name |
SPBC19F8.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACAAATTTAATATTTGCA |
| Amino acid length |
335 |
| Molecular weight |
38.5 |
| Isoelectric point (calc.) |
9.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus;
cytoplasmic dots |
| Comments for localization |
except nucleolus |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |