Gene name |
SPBC1289.07c |
Gene ID |
32/D03 |
Gene synonyms/obsolete |
rpc40; rpa42 |
Gene product |
DNA-directed RNA
polymerases (I and III subunit) |
Entry clone |
Cloned |
ORF length (unspliced) |
1047 |
ORF length (spliced) |
|
Entry clone length |
1047 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1289.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGCGGTTGACAGATC |
Rev primer name |
SPBC1289.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTCAGAAGAAATATTCTGA |
Amino acid length |
348 |
Molecular weight |
39.1 |
Isoelectric point (calc.) |
5.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus;
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |