| Gene name |
SPBC1347.03 |
| Gene ID |
32/D04 |
| Gene synonyms/obsolete |
meu14 |
| Gene product |
involved in meiosis,
spore wall assembly, nuclear division (meiosis II); predicted
coiled-coil region; meiotic expression upregulated; Mei4p
dependent transcription |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1049 |
| ORF length (spliced) |
1008 |
| Entry clone length |
1049 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1347.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCCAAATCAAGCAACTT |
| Rev primer name |
SPBC1347.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAAGAAAACAGTGGATTTT |
| Amino acid length |
335 |
| Molecular weight |
37.3 |
| Isoelectric point (calc.) |
7.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery; SPB?; site
of septum formation |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |