| Gene name |
SPBP16F5.02 |
| Gene ID |
32/E06 |
| Gene synonyms/obsolete |
mcs2 |
| Gene product |
cyclin; mitotic
catastrophe suppresso; transcription initiation factor TFIIH
subunit; interacts physically with Crk1p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1107 |
| ORF length (spliced) |
969 |
| Entry clone length |
1107 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBP16F5.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGCCGATAAATTTCG |
| Rev primer name |
SPBP16F5.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCAAACGGATTTTTATCC |
| Amino acid length |
322 |
| Molecular weight |
37.6 |
| Isoelectric point (calc.) |
8.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNALSSALSL |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |