| Gene name |
SPBC25D12.04 |
| Gene ID |
32/E12 |
| Gene synonyms/obsolete |
suc22 |
| Gene product |
ribonucleotide
reductase; ribonucleotide-diphosphate reductase (small
subunit); involved in DNA replication (required) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1176 |
| ORF length (spliced) |
|
| Entry clone length |
1176 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
7C:T / 104T:C / 700T:C
/ 709T:G / 838A:G / 933T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC25D12.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGCCTTGAGCATCTCGA |
| Rev primer name |
SPBC25D12.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAGTCCTCATCGATTGTA |
| Amino acid length |
391 |
| Molecular weight |
45.4 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |