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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC16C6.10
Gene ID 32/F02
Gene synonyms/obsolete chp2
Gene product pleiotrophic transcriptional silencing protein; transcriptional repressor; chromodomain protein; chromo shadow domain; no apparent Sc ortholog; similar to Sp swi6 (paralog)
Entry clone Cloned
ORF length (unspliced) 1180
ORF length (spliced) 1143
Entry clone length 1180
No. of intron 1
Sequence status Finished
Sequence results 83T:C / 599T:C / 821T:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC16C6.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTCAAAAGTGCCGACCT
Rev primer name SPBC16C6.10.Rv
Rev primer SEQ AGAAAGCTGGGTATGTAAATTTAATATGAGCC
Amino acid length 380
Molecular weight 42.9
Isoelectric point (calc.) 4.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 270
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) SPB; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 3 images) See all images

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