Gene name |
SPBC365.13c |
Gene ID |
32/F04 |
Gene synonyms/obsolete |
hba1; caf1 |
Gene product |
Ran-GTPase-binding
protein; involved in brefeldin A resistance; involved in
nuclear export, oxidative stress response; interacts
genetically with crm1; deletion induces nucler accumulation of
pap1p and Sty1p |
Entry clone |
Cloned |
ORF length (unspliced) |
1200 |
ORF length (spliced) |
|
Entry clone length |
1200 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
51T:C / 821A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC365.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACCAGCAAAATGGAAAA |
Rev primer name |
SPBC365.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGCATCTTCTCTTCCACCC |
Amino acid length |
399 |
Molecular weight |
43.2 |
Isoelectric point (calc.) |
5.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |