Gene name |
SPBC8D2.18c |
Gene ID |
32/G11 |
Gene synonyms/obsolete |
|
Gene product |
adenosylhomocysteinase
|
Entry clone |
Cloned |
ORF length (unspliced) |
1302 |
ORF length (spliced) |
|
Entry clone length |
1302 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
150T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC8D2.18.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTACTCAAAACTACAA |
Rev primer name |
SPBC8D2.18.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTAACGATAGTGATCAGCC |
Amino acid length |
433 |
Molecular weight |
47.3 |
Isoelectric point (calc.) |
5.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDEEVARLHL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |