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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP16F5.08c
Gene ID 32/H05
Gene synonyms/obsolete
Gene product flavin dependent monooxygenase; involved in protein folding; disulphide bond formation
Entry clone Cloned
ORF length (unspliced) 1344
ORF length (spliced)
Entry clone length 1344
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP16F5.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTGTCTTCCAACAATTCG
Rev primer name SPBP16F5.08.Rv
Rev primer SEQ AGAAAGCTGGGTACTTTGGAGGTTCAATTCCG
Amino acid length 447
Molecular weight 49.8
Isoelectric point (calc.) 7.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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