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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP16F5.06
Gene ID 33/A06
Gene synonyms/obsolete
Gene product involved in ribosome biogenesis and assembly; rrm RNA recognition motif; RNA-binding protein
Entry clone Cloned#
ORF length (unspliced) 1437
ORF length (spliced)
Entry clone length 1437
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBP16F5.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGTTAGAAAAACGGAT
Rev primer name SPBP16F5.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAACAAGTCGAATACGCTTT
Amino acid length 478
Molecular weight 54.8
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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