Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBC543.06c
Gene ID 33/B02
Gene synonyms/obsolete
Gene product DEAD/DEAH box helicase; ATP-dependent; RNA helicase; involved in ribosome biogenesis and assembly
Entry clone Cloned
ORF length (unspliced) 1518
ORF length (spliced) 1362
Entry clone length 1518
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC543.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAGAACATACAAGAAA
Rev primer name SPBC543.06.Rv
Rev primer SEQ AGAAAGCTGGGTACTTTTCCGTAGATAAAGTA
Amino acid length 453
Molecular weight 50.7
Isoelectric point (calc.) 8.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LIDTLKALAI/LIYSILRLLEL
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.