| Gene name |
SPBC16A3.01 |
| Gene ID |
33/B05 |
| Gene synonyms/obsolete |
spn3;
SPBC543.01c |
| Gene product |
septin; involved in
cytokinesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1528 |
| ORF length (spliced) |
1239 |
| Entry clone length |
1528 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC16A3.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTGGTATACATATAACGA |
| Rev primer name |
SPBC16A3.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGACTGGGAATGATTAGTT |
| Amino acid length |
412 |
| Molecular weight |
46.6 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery at site of
septum formation; cytosol; a few cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |