Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC8D2.13
Gene ID 33/C02
Gene synonyms/obsolete pi052
Gene product involved in snoRNP biogenesis
Entry clone Cloned
ORF length (unspliced) 1583
ORF length (spliced) 1356
Entry clone length 1583
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC8D2.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATCACTCCAAAATTTGA
Rev primer name SPBC8D2.13.Rv
Rev primer SEQ AGAAAGCTGGGTATTTCTCTATGGACTCGCTT
Amino acid length 451
Molecular weight 52.3
Isoelectric point (calc.) 4.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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