Gene name |
SPBC21D10.04c |
Gene ID |
33/C04 |
Gene synonyms/obsolete |
fnx1;
SPBC12C2.13c |
Gene product |
MFS multidrug efflux
transporter; multidrug resistance protein; involved in the
transition of cells to a quiescent G0 state |
Entry clone |
Cloned |
ORF length (unspliced) |
1596 |
ORF length (spliced) |
|
Entry clone length |
1596 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC21D10.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTCGATCAGGTAAATTT |
Rev primer name |
SPBC21D10.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGAACGATCAACAGAGGTA |
Amino acid length |
531 |
Molecular weight |
58 |
Isoelectric point (calc.) |
9.2 |
Signal SEQ |
|
No. of transmembrane domain |
14 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPKDIQKLVI |
Localization (YFP) |
vacuole membrane |
Comments for localization |
aggregates by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |