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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC21D10.04c
Gene ID 33/C04
Gene synonyms/obsolete fnx1; SPBC12C2.13c
Gene product MFS multidrug efflux transporter; multidrug resistance protein; involved in the transition of cells to a quiescent G0 state
Entry clone Cloned
ORF length (unspliced) 1596
ORF length (spliced)
Entry clone length 1596
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC21D10.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTCGATCAGGTAAATTT
Rev primer name SPBC21D10.04.Rv
Rev primer SEQ AGAAAGCTGGGTATGAACGATCAACAGAGGTA
Amino acid length 531
Molecular weight 58
Isoelectric point (calc.) 9.2
Signal SEQ
No. of transmembrane domain 14
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LPKDIQKLVI
Localization (YFP) vacuole membrane
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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