Gene name |
SPBC685.09 |
Gene ID |
33/C05 |
Gene synonyms/obsolete |
orp2; orc2 |
Gene product |
origin recognition
complex |
Entry clone |
Cloned |
ORF length (unspliced) |
1608 |
ORF length (spliced) |
|
Entry clone length |
1608 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC685.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTAATAAACGAGCACGT |
Rev primer name |
SPBC685.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATACGTCTTCCATCATATCC |
Amino acid length |
535 |
Molecular weight |
61 |
Isoelectric point (calc.) |
5.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
periphery; nuclear
envelope with projection by over expression; periphery at site
of septum formation; SPB |
Comments for localization |
anaphase SPB |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |