| Gene name |
SPBC1604.05 |
| Gene ID |
33/C10 |
| Gene synonyms/obsolete |
pgi1 |
| Gene product |
glucose-6-phosphate
isomerase; involved in gluconeogenesis; involved in
glycolysis; involved in pentose-phosphate shunt;
glucose-6-phosphate isomerase activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1653 |
| ORF length (spliced) |
|
| Entry clone length |
1653 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1604.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTTTAGCTTAGCTTC |
| Rev primer name |
SPBC1604.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAATCCATTCTTGAAAAGG |
| Amino acid length |
550 |
| Molecular weight |
60.8 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol; cytoplasmic
dots; Golgi? |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |