| Gene name |
SPBC8D2.01 |
| Gene ID |
33/D04 |
| Gene synonyms/obsolete |
gsk31; pi064 |
| Gene product |
serine/threonine
protein kinase; glycogen synthase kinase 3 (GSK-3) family;
similar to Sp gsk3 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1748 |
| ORF length (spliced) |
1146 |
| Entry clone length |
1748 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
736T:deletion / 739T:C
/ 1355T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC8D2.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTGGTAAATAAGAAAA |
| Rev primer name |
SPBC8D2.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGAGTCTGCGTCAGCCTGT |
| Amino acid length |
381 |
| Molecular weight |
43.3 |
| Isoelectric point (calc.) |
7.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
115 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHRVNRELQI |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |