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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP26C9.03c
Gene ID 33/D06
Gene synonyms/obsolete
Gene product metal permease
Entry clone Cloned
ORF length (unspliced) 1755
ORF length (spliced)
Entry clone length 1755
No. of intron 0
Sequence status Finished
Sequence results 544C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP26C9.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGCCTCTATCACAGA
Rev primer name SPBP26C9.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAACAGTGCTGGTGGTTGAA
Amino acid length 584
Molecular weight 66
Isoelectric point (calc.) 6
Signal SEQ
No. of transmembrane domain 7
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFSVFVILGL
Localization (YFP) Golgi; periphery at cell tip and site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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