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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC8F11.07c
Gene ID 33/E03
Gene synonyms/obsolete cdc24
Gene product involved in DNA replication (required); essential; no apparent orthologs
Entry clone Cloned in 2006 trial
ORF length (unspliced) 1823
ORF length (spliced) 1506
Entry clone length 1823
No. of intron 6
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC8F11.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTTTCCAGGTCTGAT
Rev primer name SPAC8F11.07.Rv
Rev primer SEQ AGAAAGCTGGGTATTCACACGGCAGAGAGTTT
Amino acid length 501
Molecular weight 58
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFSGVSGHLKI
Localization (YFP) nucleus>cytosol; site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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