Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC2D10.18
Gene ID 33/E04
Gene synonyms/obsolete abc1
Gene product involved in assembly of the ubiquinol cytochrome-c reductase complex (cytochrome bc1 complex); functionally complements Sc ABC1
Entry clone Cloned
ORF length (unspliced) 1833
ORF length (spliced)
Entry clone length 1833
No. of intron 0
Sequence status Finished
Sequence results 231T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC2D10.18.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTAATTCAGGGCTTTG
Rev primer name SPBC2D10.18.Rv
Rev primer SEQ AGAAAGCTGGGTAATCAGCGTAATGTTTCAAC
Amino acid length 610
Molecular weight 68.5
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain 17
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion; periphery
Comments for localization weak signal of periphery
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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