| Gene name |
SPBC8D2.19 |
| Gene ID |
33/E07 |
| Gene synonyms/obsolete |
mde3 |
| Gene product |
serine/threonine
protein kinase; positive regulator of meiotic genes; meiosis
specific transcription |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
1859 |
| ORF length (spliced) |
1680 |
| Entry clone length |
1859 |
| No. of intron |
2 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC8D2.19.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTAATGAAAGTATTTA |
| Rev primer name |
SPBC8D2.19.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGGCGAACTTAGATGTAAG |
| Amino acid length |
559 |
| Molecular weight |
63.2 |
| Isoelectric point (calc.) |
9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLKLRESLAL |
| Localization (YFP) |
periphery, especially
at cell tip and site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |