| Gene name |
SPBP35G2.07 |
| Gene ID |
33/F03 |
| Gene synonyms/obsolete |
ilv1 |
| Gene product |
acetolactate synthase
subunit; valine and isoleucine biosynthesis pathway (1st
step); functionally complements Sc ILV2 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2010 |
| ORF length (spliced) |
|
| Entry clone length |
2010 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
327A:G / 1396G:A /
1991T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBP35G2.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTGTGCTGGCTCCCCT |
| Rev primer name |
SPBP35G2.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGAAAGGCTTTCATGCAAA |
| Amino acid length |
669 |
| Molecular weight |
73.1 |
| Isoelectric point (calc.) |
9.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLRKFSERLQI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |