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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC29B5.02c
Gene ID 33/G09
Gene synonyms/obsolete isp4
Gene product involved in sexual differentiation; OPT oligopeptide transporter family
Entry clone Cloned
ORF length (unspliced) 2358
ORF length (spliced)
Entry clone length 2358
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC29B5.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATCGGTTCCATCAATGA
Rev primer name SPBC29B5.02.Rv
Rev primer SEQ AGAAAGCTGGGTACCATTCGGCGGGACCAAAG
Amino acid length 785
Molecular weight 89.1
Isoelectric point (calc.) 5.9
Signal SEQ
No. of transmembrane domain 14
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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