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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC4.04c
Gene ID 33/H09
Gene synonyms/obsolete mcm2; nda1; cdc19
Gene product MCM complex subunit; involved in DNA replication (initiation) (required); involved in cytokinesis (required); involved in septation (required); expression peaks at M-G1 phase; regulated by PBF transcription complex
Entry clone Cloned
ORF length (unspliced) 2712
ORF length (spliced) 2493
Entry clone length 2712
No. of intron 1
Sequence status Finished
Sequence results 556G:A / 1127G:A / 1813T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC4.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTCTTTTCGGAAAAG
Rev primer name SPBC4.04.Rv
Rev primer SEQ AGAAAGCTGGGTAAATAAGATATTTAGCAAAT
Amino acid length 830
Molecular weight 92.8
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: nucleus>>cytosol
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images
LMB 4 images) See all images

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