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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP23A10.13
Gene ID 34/A02
Gene synonyms/obsolete orc4; orp4
Gene product origin recognition complex (subunit 4); binds to replication origin DNA via multiple AT-hooks; AAA family ATPase
Entry clone Cloned in 2004 trial
ORF length (unspliced) 2919
ORF length (spliced)
Entry clone length 2919
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBP23A10.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCTCATCCCCATTCAC
Rev primer name SPBP23A10.13.Rv
Rev primer SEQ AGAAAGCTGGGTATATAACCTCCTTAAGCCAG
Amino acid length 972
Molecular weight 108.6
Isoelectric point (calc.) 9.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 101/200
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LREISRQLSI/LVKNLSLLEL
Localization (YFP) nucleus; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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