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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC3E7.08c
Gene ID 34/A07
Gene synonyms/obsolete rad13
Gene product DNA repair nuclease; involved in DNA repair; involved in nucleotide-excision repair; XP-G family
Entry clone Cloned
ORF length (unspliced) 3339
ORF length (spliced)
Entry clone length 3339
No. of intron 0
Sequence status Finished
Sequence results 22G:A / 2872T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC3E7.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGGGTTTCAGGTCTTTG
Rev primer name SPBC3E7.08.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTCTTCTTCTTTTGCTG
Amino acid length 1112
Molecular weight 126.3
Isoelectric point (calc.) 4.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LETLVNKRLAI/LSRVGPVLAL
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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