Gene name |
SPBC3E7.08c |
Gene ID |
34/A07 |
Gene synonyms/obsolete |
rad13 |
Gene product |
DNA repair nuclease;
involved in DNA repair; involved in nucleotide-excision
repair; XP-G family |
Entry clone |
Cloned |
ORF length (unspliced) |
3339 |
ORF length (spliced) |
|
Entry clone length |
3339 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
22G:A / 2872T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC3E7.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGGTTTCAGGTCTTTG |
Rev primer name |
SPBC3E7.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTCTTCTTCTTTTGCTG |
Amino acid length |
1112 |
Molecular weight |
126.3 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LETLVNKRLAI/LSRVGPVLAL |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |