Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP4H10.06c
Gene ID 34/A08
Gene synonyms/obsolete cut14
Gene product condensin subunit; SMC2 subunit; essential; involved in chromosome condensation (mitosis) (required); involved in chromosome segregation (mitosis) (required)
Entry clone Cloned in 2006 trial
ORF length (unspliced) 3519
ORF length (spliced)
Entry clone length 3519
No. of intron 0
Sequence status Partially sequenced
Sequence results 100% match in both ends
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBP4H10.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAATAGAGGAACTCAT
Rev primer name SPBP4H10.06.Rv
Rev primer SEQ AGAAAGCTGGGTAGCGAGCTTGTACCACAGAT
Amino acid length 1172
Molecular weight 134.1
Isoelectric point (calc.) 8.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDAICFVLGI/LPELDRLIL/LDEIDAALDL
Localization (YFP) no expression clone
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation

Image information
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