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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC146.03c
Gene ID 34/A12
Gene synonyms/obsolete cut3
Gene product condensin subunit; SMC4 subunit; essential; involved in chromosome segregation (required) (mitosis); involved in chromosome condensation (required) (mitosis); NTP binding motif; phosphorylated by Cdc2p
Entry clone Cloned
ORF length (unspliced) 3975
ORF length (spliced)
Entry clone length 3975
No. of intron 0
Sequence status Finished
Sequence results 1550A:G / 1736T:C / 3051T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC146.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGACAAGGGCATCTT
Rev primer name SPBC146.03.Rv
Rev primer SEQ AGAAAGCTGGGTAATCTGTAAGTATTTCCTTG
Amino acid length 1324
Molecular weight 150.6
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LNSEIADLSL/LKLMSNLKL
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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