Gene name |
SPBC16D10.05 |
Gene ID |
34/B09 |
Gene synonyms/obsolete |
mok13 |
Gene product |
a-1,3-glucan synthase;
alpha glucan synthase; glycosyl transferase family 1; similar
to Sp MOK1 and MOK11 and MOK14 and MOK12; involved in cell
wall biosynthesis; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
7117 |
ORF length (spliced) |
7077 |
Entry clone length |
7117 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
237A:G / 4790G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16D10.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGAAATAAGAATATTCT |
Rev primer name |
SPBC16D10.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGGCCGACTTAAATTTTCT |
Amino acid length |
2358 |
Molecular weight |
269.1 |
Isoelectric point (calc.) |
7 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
13 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKDSLDYLEI/LPNVIPCVLSL/LPSVASLSL/LIAVFSWPLAL/LGPGLVFLDL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |