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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4A8.05c
Gene ID 34/C05
Gene synonyms/obsolete myo3
Gene product myosin-3 isoform, heavy chain (Type II myosin); involved in cytokinesis; involved in contractile ring assembly; non-essential
Entry clone Cloned
ORF length (unspliced) 6315
ORF length (spliced)
Entry clone length 6315
No. of intron 0
Sequence status Finished
Sequence results 3573T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC4A8.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGTATCTTTCAAAAAA
Rev primer name SPAC4A8.05.Rv
Rev primer SEQ AGAAAGCTGGGTACCTTAGAACGCTAGGCGAA
Amino acid length 2104
Molecular weight 242.5
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 1156/1157
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFLIIASILHI/LQHNLRQLKL/LMRLDDELLL/LELKLFDLDL
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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