Gene name |
SPBC16C6.02c |
Gene ID |
34/D07 |
Gene synonyms/obsolete |
|
Gene product |
involved in
intracellular protein transport |
Entry clone |
Cloned |
ORF length (unspliced) |
9808 |
ORF length (spliced) |
9396 |
Entry clone length |
9808 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
1757C:T / 4432A:G /
7406T:C / 9782T:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16C6.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTAGAAGGGTTACTAGC |
Rev primer name |
SPBC16C6.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACGGTTCGCTTGAGGAACTA |
Amino acid length |
3131 |
Molecular weight |
354 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDVLELNLDI/LAKFSILTL/LHCGISELLI/LASNLPEMLSI/LIKMASLAI/LACLYPLRI/LQLNILPTLQI/LSLEVEDRLVL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |