| Gene name |
SPAC9.07c |
| Gene ID |
34/E01 |
| Gene synonyms/obsolete |
|
| Gene product |
GTP-binding protein;
TGS domain; GTP1/OBG family |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1199 |
| ORF length (spliced) |
1101 |
| Entry clone length |
1199 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC9.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGACTACGGCGCAAAA |
| Rev primer name |
SPAC9.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTTTGACAATCGTCACT |
| Amino acid length |
366 |
| Molecular weight |
40.3 |
| Isoelectric point (calc.) |
8.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKETMWDYLNL |
| Localization (YFP) |
nucleus>=cytosol |
| Comments for localization |
cytoplasmic dots by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |