Gene name |
SPCC794.04c |
Gene ID |
34/E11 |
Gene synonyms/obsolete |
|
Gene product |
unknown specificity;
transporter; similar to Sp SPAC11D3.05 and SPBC530.15C and
SPCC569.05C and SPBC36.03C and SPBC36.01C and SPBC36.02C and
SPBC530.02 and SPBC947.06C; involved in amine/polyamine
transport |
Entry clone |
Cloned |
ORF length (unspliced) |
1644 |
ORF length (spliced) |
|
Entry clone length |
1644 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
657T:C / 1200T:C /
1335G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC794.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTACATTTTCTTAAT |
Rev primer name |
SPCC794.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCCTTTATAAGCATGAGGA |
Amino acid length |
547 |
Molecular weight |
61.7 |
Isoelectric point (calc.) |
7.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSEVYGRLPL/LVFINILLYI/LGILVGILLGL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Confocal |