| Gene name |
SPAC30.05 |
| Gene ID |
34/F01 |
| Gene synonyms/obsolete |
pof1;
SPAC29E6.01 |
| Gene product |
F-box protein; WD
repeat protein; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1647 |
| ORF length (spliced) |
1521 |
| Entry clone length |
1647 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
95T:C / 1562A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC30.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACCAGGACGACAATGG |
| Rev primer name |
SPAC30.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAAAATGTACAATCCAAT |
| Amino acid length |
506 |
| Molecular weight |
58.2 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |