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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17C9.16c
Gene ID 34/F06
Gene synonyms/obsolete SPAC9E9.16
Gene product membrane transporter; unknown specificity; similar to Sp SPBC609.04 (paralog)
Entry clone Cloned
ORF length (unspliced) 1649
ORF length (spliced) 1596
Entry clone length 1649
No. of intron 1
Sequence status Finished
Sequence results 423A:G / 491T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17C9.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTGGTTTTTAAGAGAAAC
Rev primer name SPAC17C9.16.Rv
Rev primer SEQ AGAAAGCTGGGTAAGTGGCATATTTTGATCGA
Amino acid length 531
Molecular weight 59.3
Isoelectric point (calc.) 7.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSLIVFICLQI/LLMMISALVL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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