Gene name |
SPAC26F1.10c |
Gene ID |
34/F11 |
Gene synonyms/obsolete |
pyp1 |
Gene product |
protein-tyrosine
phosphatase; negative regulator of mitosis; negative regulator
of nutrient monitoring pathways; dephosphorylate an
osmosensing MAP kinase controlling cell size at division;
similar to Sp pyp2 |
Entry clone |
Cloned |
ORF length (unspliced) |
1653 |
ORF length (spliced) |
|
Entry clone length |
1653 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC26F1.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTTTTCAAACGGTTC |
Rev primer name |
SPAC26F1.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGTTAAAACCGGGAAATGA |
Amino acid length |
550 |
Molecular weight |
61.5 |
Isoelectric point (calc.) |
8.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica;
DeltaVision |