| Gene name |
SPBC28F2.10c |
| Gene ID |
34/G03 |
| Gene synonyms/obsolete |
kap1 |
| Gene product |
kinesin-associated
protein; SAGA complex (Spt-Ada-Gcn5-Acetyltransferase); ADA
complex |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1656 |
| ORF length (spliced) |
|
| Entry clone length |
1656 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1639T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC28F2.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCTCCGAACAACAAAA |
| Rev primer name |
SPBC28F2.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACAGATGTACTGTAATTA |
| Amino acid length |
551 |
| Molecular weight |
62.4 |
| Isoelectric point (calc.) |
4.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
473 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRYTLKQLGI |
| Localization (YFP) |
nucleus; nuclear
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |