Gene name |
SPBC409.11 |
Gene ID |
34/G12 |
Gene synonyms/obsolete |
meu18 |
Gene product |
predicted coiled-coil;
NLS; meiotic expression upregulated; no apparent
orthologs |
Entry clone |
Cloned |
ORF length (unspliced) |
1662 |
ORF length (spliced) |
|
Entry clone length |
1662 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
661A:G / 903A:G /
1085G:C / 1101A:T / 1278A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC409.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTTACATAAACTTTTA |
Rev primer name |
SPBC409.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATACCAACAAATTGGTCTCC |
Amino acid length |
553 |
Molecular weight |
64.7 |
Isoelectric point (calc.) |
8.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
289 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIPSFHVCLLI/LLYCFHYALDL/LTTIGNNLRI |
Localization (YFP) |
nuclear envelope;
periphery; cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |