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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC19G7.06
Gene ID 34/H04
Gene synonyms/obsolete
Gene product MAD box transcription factor; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1664
ORF length (spliced) 1311
Entry clone length 1664
No. of intron 3
Sequence status Finished
Sequence results 516G:A / 1502G:C / 1513T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC19G7.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATATTAATCCTCCTCC
Rev primer name SPBC19G7.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGGGCATTTCGTTCAATA
Amino acid length 436
Molecular weight 48.3
Isoelectric point (calc.) 5.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus>>cytosol; nuclear dots)
Microscope used for observation Leica

Image information
YFP 4 images) See all images
LMB 4 images) See all images

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