Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC30D11.08c
Gene ID 34/H08
Gene synonyms/obsolete
Gene product zinc finger protein; zf-PHD finger; involved in transcriptional regulation; similar to Sp SPCC4G3.07C (paralog)
Entry clone Cloned
ORF length (unspliced) 1666
ORF length (spliced) 1617
Entry clone length 1666
No. of intron 1
Sequence status Finished
Sequence results 314A:T / 515A:G / 1371T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC30D11.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCGAATTCATCGTACTA
Rev primer name SPAC30D11.08.Rv
Rev primer SEQ AGAAAGCTGGGTAAATACTACTAAGAACAATT
Amino acid length 538
Molecular weight 60.6
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 3 images) See all images

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